high plex core image 2024

High-Plex Immunocytochemistry Core

Major Core Instrument / Services

The Core utilizes the Akoya PhenoCycler-Fusion to experience the fastest spatial biology solution.

Akoya Phenocycler

Akoya PhenoCycler-Fusion

    • 30+ antibodies optimized on FFPE in human brain tissue
    • 8 antibodies optimized in mouse brain FFPEs
    • 11 antibodies optimized in frozen brain tissues from mice
    • Developed and implemented a photobleaching method to minimize autofluorescence, enhancing marker signals
    • Getting ready as a Recharge Core
high plex core image, 2024

Core Highlights

2024 started with the Core’s first publication using the technology to perform feasibility tests to characterize tumor immune microenvironment changes in patients from a University of Michigan clinical trial that utilized a multi-pronged immunotherapy approach for the treatment of glioblastoma. This publication in The Lancet Oncology (Umemura et al., 2023) demonstrated the power of our workflow, which utilizes conventional tissue slides to map numerous cell types in space. Combining protein expression data with spatial context allows us to explore new frontiers in single cell spatial analysis, like changes in cell neighborhoods and cell-cell interactions between experimental groups.

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Contact

CORE EMAIL :  HIGHPLEXLCCORE@UMICH.EDU