Upcoming Publication (In Press): Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics

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Upcoming publication in Methods in Cell Biology

Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics

Mahmoud S. Alghamri, Brandon L. McClellan, Kaushik Banerjee, Jorge A. Peña Agudelo, Pedro R. Lowenstein, and Maria G. Castro*

This chapter outlines key in vitro assays to study T cell proliferation, inhibitory marker expression, and effector function under immunosuppressive conditions. It also demonstrates how these assays can evaluate immunotherapies aimed at restoring T cell activity within the tumor microenvironment.

Access the full chapter here once it is published: https://doi.org/10.1016/bs.mcb.2025.03.009